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U of T researchers have discovered that a single molecular event in our cells could hold the key to how we evolved to become the smartest creatures on the planet.

Professor and his team at the have determined that a small change in a protein called PTBP1 spurred the creation of neurons and fuelled the evolution of mammalian brains to become the largest and most complex among vertebrates.

The study is published in . 

Brain size and complexity vary enormously across vertebrates, but it is not clear how these differences came about. Humans and frogs, for example, have been evolving separately for 350 million years and have very different brain abilities. Yet scientists have shown that they use a remarkably similar repertoire of genes to build organs in the body.

So how is it that a similar number of genes, which are also switched on or off in similar ways in diverse vertebrate species, generate a vast range of organ size and complexity?

The key lies in alternative splicing (AS), whereby gene products are assembled into proteins, which are the building blocks of life. During AS, gene fragments called exons are shuffled to make different protein shapes. It’s like LEGO, where some fragments can be missing from the final protein shape.

AS enables cells to make more than one protein from a single gene, so that the total number of different proteins in a cell greatly surpasses the number of available genes. A cell’s ability to regulate protein diversity at any given time reflects its ability to take on different roles in the body.

Blencowe’s previous work showed that AS prevalence increases with vertebrate complexity. So although the genes that make bodies of vertebrates might be similar, the proteins they give rise to are far more diverse in mammals than in birds and frogs.

And nowhere is AS more widespread than in the brain.

“We wanted to see if AS could drive morphological differences in the brains of different vertebrate species,” says graduate student Serge Gueroussov, lead author of the study. Gueroussov previously helped identify PTBP1 as a protein that takes on another form in mammals. The mammalian form of PTBP1 is shorter because a small fragment is omitted during AS and does not make it into the final protein shape.
 
Could this newly acquired, mammalian version of PTBP1 provide clues to how our brains evolved? PTBP1 is both a target and major regulator of AS. PTBP1’s job in a cell is to stop it from becoming a neuron by holding off AS of hundreds of other gene products.

Gueroussov showed that in mammalian cells, the presence of the shorter version of PTBP1 unleashes a cascade of AS events, tipping the scales of protein balance so that a cell becomes a neuron.

What’s more, when Gueroussov engineered chicken cells to make the shorter, mammalian-like PTBP1, this triggered AS events that are found in mammals.

“One interesting implication of our work is that this particular switch between the two versions of PTBP1 could have affected the timing of when neurons are made in the embryo in a way that creates differences in morphological complexity and brain size,” says Blencowe, who is also Banbury Chair in Medical Research and a professor in the department of molecular genetics.

(Image at right: frog and human brain, to scale.)

As scientists continue to sift through countless molecular events occurring in our cells, they will keep finding clues as to how our bodies and minds came to be.

“This is the tip of an iceberg in terms of the full repertoire of AS changes that likely have contributed major roles in driving evolutionary differences,” says Blencowe.

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